Publications
2020
Birgit Sawitzki, Paul N Harden, Petra Reinke, Aurélie Moreau, James A Hutchinson, David S Game, Qizhi Tang, Eva C Guinan, Manuela Battaglia, William J Burlingham, Ian S D Roberts, Mathias Streitz, Régis Josien, Carsten A Böger, Cristiano Scottà, James F Markmann, Joanna L Hester, Karsten Juerchott, Cecile Braudeau, Ben James, Laura Contreras-Ruiz, Jeroen B van der Net, Tobias Bergler, Rossana Caldara, William Petchey, Matthias Edinger, Nathalie Dupas, Michael Kapinsky, Ingrid Mutzbauer, Natalie M Otto, Robert Öllinger, Maria P Hernandez-Fuentes, Fadi Issa, Norbert Ahrens, Christoph Meyenberg, Sandra Karitzky, Ulrich Kunzendorf, Stuart J Knechtle, Josep Grinyó, Peter J Morris, Leslie Brent, Andrew Bushell, Laurence A Turka, Jeffrey A Bluestone, Robert I Lechler, Hans J Schlitt, Maria C Cuturi, Stephan Schlickeiser, Peter J Friend, Tewfik Miloud, Alexander Scheffold, Antonio Secchi, Kerry Crisalli, Sang-Mo Kang, Rachel Hilton, Bernhard Banas, Gilles Blancho, Hans-Dieter Volk, Giovanna Lombardi, Kathryn J Wood, Edward K Geissler. Regulatory cell therapy in kidney Transplantation (The ONE Study): A harmonised design and analysis of seven non-randomised, single-arm, phase 1/2A trials. Lancet 2020 May 23;395(10237):1627-1639.
2014
Broichhausen C, Riquelme P, Ahrens N, Wege A, Koehl G, Schlitt HJ, Banas B, Fändrich F, Geissler EK, and Hutchinson JA. In question: The scientific value of preclinical safety pharmacology and toxicology studies with cell-base therapies. Mol Ther 2014; 1,14026; doi: 10.1038/mtm.2014.26.
Edozie FC, Nova-Lamperti EA, Povoleri GA, Scottà C, John S, Lombardi G, Afzali B. Regulatory T-cell therapy in the induction of transplant tolerance: the issue of subpopulations. Transplantation. 2014 98(4): 370-9.
Hutchinson JA, Ahrens N, Riquelme P, Walter L, Gruber M, Böger CA, Farkas S, Scherer MN, Broichhausen C, Bein T, Schlitt HJ, Fändrich F, Banas B, Geissler EK. Clinical management of patients receiving cell-based immunoregulatory therapy. Transfusion 2014 54 (9):2336-2343.
Lantow M, Eggenhofer E, Sabet-Baktach M, Renner P, Rovira J, Koehl GE, Schlitt HJ, Geissler EK, Kroemer A. CD27low NK cells prolong allograft survival in mice by controlling alloreactive CD8+ T cells in a T-bet dependent manner. Transplantation, in press
Landwehr-Kenzel S, Issa F, Luu SH, Schmück M, Lei H, Zobel A, Thiel A, Babel N, Wood K, Volk HD, Reinke P. Novel GMP-Compatible Protocol Employing an Allogeneic B Cell Bank for Clonal Expansion of Allospecific Natural Regulatory T Cells.
Am J Transplant. 2014 Mar;14(3):594-606
Lee K, Nguyen V, Lee KM, Kang SM, Tang Q. Attenuation of donor-reactive T cells allows effective control of allograft rejection using regulatory T cell therapy. Am J Transplant. 2014 Jan;14(1):27-38.
Malaisé M, Rovira J, Renner P, Eggenhofer E, Sabet-Baktach M, Lantow M, Lang SA, Koehl GE, Farkas SA, Loss M, Agha A, Campistol JM, Schlitt HJ, Geissler EK, Kroemer A. KLRG1+ NK Cells Protect T-bet-deficient mice from pulmonary metastatic colorectal carcinoma. J Immunol 2014 192:1954-1961
Managh A, Hutchinson RW, Riquelme P, Broichhausen C, Wege AK, Ritter U, Ahrens N, Koehl GE, Walter L, Schlitt HJ, Reid HJ, Geissler EK, Sharp BL, Hutchinson JA. Laser-ablation inductively-coupled plasma mass spectrometry: An emerging technology for detecting rare cells in tissue sections. J Immunol 2014; 193(5):2600-2608.
McGregor R, Li G, Penny H, Lombardi G, Afzali B, Goldsmith DJ. Vitamin D in renal transplantation- from biological mechanisms to clinical benefits Am J Transplant. 2014 14(6):1259-70
Moreau A., Blair P. A., Chai J., Ratnasothy K., Stolarczyk E., Alhabbab R., Rackham C. L., Jones P. M., Smyth L., Elgueta R., Howard J. K., Lechler R. I., and Lombardi G. Transitional-2 B cells acquire regulatory function during tolerance induction and contribute to allograft survival. Eur J Immunol.2014 Nov 19. doi: 10.1002/eji.201445082. [Epub ahead of print].
Renner P, Rovira J, Klein C, Schlitt HJ, Geissler EK, Kroemer A. KLRG1+ natural killer cells protect against pulmonary metastatic disease by immunosurveillance. Oncoimmunology 2014 Apr 8;3:e28328 eCollectionSharif-Paghaleh E, Leech J, Sunassee K, Ali N, Sagoo P, Lechler RI, Smyth LA, Lombardi G, Mullen GE. Monitoring the efficacy of dendritic cell vaccination by early detection of (99m) Tc-HMPAO-labelled CD4(+)T cells. Eur J Immunol. 2014 44(7):2188-91
Smyth LA, Boardman D, Tung S, Lechler R, Lombardi G. MicroRNAs affect dendritic cells function and phenotype. Immunology. 2014 Sep 20. doi: 10.1111/imm.12390. Tang Q Pharmacokinetics of therapeutic Tregs Am J Transpl. 2014 14:2679-2680Tang Q, Kang SM. Interpretation of transplant biopsies and immune responses following Treg cell therapy. Curr Opin Organ Transplant. 2014 Dec;19(6):616-20.
Xiao F, Ma L, Zhao M, Huang G, Mirenda V, Dorling A, Lechler R, Lombardi G.Ex vivo expanded human regulatory T cells delay islet allograft rejection via inhibiting islet-derived monocytechemoattractant protein-1 production in CD34+ stem cells-reconstituted NOD-scid IL2rγnull micePLoS One. 2014 9(3):e90387. doi: 10.1371/journal.pone.0090387. eCollection 2014.
Zimara N, Florian C, Schmid M, Malissen B, Kissenpfennig A, Männel DN, Edinger M, Hutchinson JA, Hoffmann P and Ritter U. Langerhans cells promote early germinal center formation in response to Leishmania-derived cutaneous antigens. Eur J Immunol 2014; 44(10):2955-67.
2013
Abou-El-Enein M1, Römhild A, Kaiser D, Beier C, Bauer G, Volk HD, Reinke P.
Good Manufacturing Practices (GMP) manufacturing of advanced therapy medicinal products: a novel tailored model for optimizing performance and estimating costs.
Cytotherapy. 2013 Mar;15(3):362-83. doi: 10.1016/j.jcyt.2012.09.006.
Afzali B., P.J. Mitchell, F.C. Edozie, G.A. Povoleri, S.E. Dowson, L. Demandt, G. Walter, J.B. Canavan, C. Scotta, B. Menon, P.S. Chana, W. Khamri, S.Y. Kordasti, S. Heck, B. Grimbacher, T. Tree, A.P. Cope, L.S. Taams, R.I. Lechler, S. John, G. Lombardi. CD161 expression characterize a subpopulation of human regulatory T cells that produce IL-17 in a STAT3-dependent manner. Eur. Immunol. 2013 43: 2043-2054.
Bacher P, Kniemeyer O, Schönbrunn A, Sawitzki B, Assenmacher M, Rietschel E, Steinbach A, Cornely OA, Brakhage AA, Thiel A, Scheffold A. Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol. 2013 Dec 4. doi: 10.1038/mi.2013.107.Canavan. J.B., B. Afzali, G.M. Lord, G. Lombardi. Assessment of regulatory T cell function in forthcoming clinical trials of cell therapy. Expert Rev Mol Diagn. 2013 13: 5-7.
Chowdhury P. and M.P. Hernandez-Fuentes Non-invasive biomarkers to guide management following renal transplantation: the need for a multiplatform approach.
Curr Opin Organ Transplant. 2013 18:1-5.
Dziubianau M1, Hecht J, Kuchenbecker L, Sattler A, Stervbo U, Rödelsperger C, Nickel P, Neumann AU, Robinson PN, Mundlos S, Volk HD, Thiel A, Reinke P, Babel N. TCR repertoire analysis by next generation sequencing allows complex differential diagnosis of T cell-related pathology Am J Transplant. 2013 Nov;13(11):2842-54. doi: 10.1111/ajt.12431. Epub 2013 Sep 10.
Eggenhofer E, Rovira J, Sabet-Baktach M, Groell A, Scherer MN, Dahlke MH, Farkas SA, Loss M, Koehl GE, Lang SA, Melter M, Schlitt HJ, Geissler EK, Kroemer A. Unconventional RORgt+ T cells drive hepatic ischemia reperfusion injury. J Immunol 2013 191:480-487.
Geissler EK and Hutchinson JA. Cell therapy as a strategy to minimize maintenance immunosuppression in solid organ transplant recipients Curr Opin Organ Transplant. 2013 Aug;18(4):408-15. doi:10.1097/MOT.0b013e328363319d.
Gerlach UA, Vogt K, Schlickeiser S, Meisel C, Streitz M, Kunkel D, Appelt C, Ahrlich S, Lachmann N, Neuhaus P, Pascher A, Sawitzki B. Elevation of CD4+ differentiated memory T cells is associated with acute cellular and antibody-mediated rejection after liver transplantation. Transplantation 2013 95(12):1512-20.Leech JM, Sharif-Paghaleh E, Maher J, Livieratos L, Lechler RI, Mullen GE, Lombardi G, Smyth LA. Whole body imaging of adoptively transferred T cells using magnetic resonance imaging, single photon emission computed tomography and positron emission tomography techniques, with focus on regulatory T cells. Clin Exp Immunol. 2013 172: 158-168.
Managh A.J., Edwards S.L., Bushell A., Wood K.J., Geissler E.K., Hutchinson J.A., Hutchinson R.W., Reid H.J., Sharp B.L. Single Cell Tracking of Gadolinium Labeled CD4+ T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry. Anal Chem. 2013, 85(22):10627-10634.
Povoleri GA, Scottà C, Nova-Lamperti EA, John S, Lombardi G, Afzali B. Thymic versus induced regulatory T cells – who regulates the regulators?Front Immunol. 2013 4:169 doi: 10.3389/fimmu.2013.00169.
Putnam A. L., N. Safinia, A. Medvec, M. Laszkowska, M. Wray, M. A. Mintz, E. Trotta, G. L. Szot, W. Liu, A. Lares, K. Lee, A. Laing, R. I. Lechler, J. L. Riley, J. A. Bluestone, G. Lombardi, Q. Tang. Clinical grade manufacturing of human alloantigen-reactive regulatory T cells for use in transplantation. Am J Transpl. 2013 13: 3010-3020.
Refik Gökmen M. and M. P. Hernandez-Fuentes. Biomarkers of Tolerance. Curr Opin Organ Transplant. 2013 18: 416–420.
Riquelme P, Tomiuk S, Kammler A, Fändrich F, Schlitt HJ, Geissler EK, Hutchinson JA. IFN-γ-induced iNOS Expression in Mouse Regulatory Macrophages Prolongs Allograft Survival in Fully Immunocompetent Recipients. Mol Ther. 2013 21, 409-422
Sabet-Baktach M, Eggenhofer E, Rovira J, Renner P, Lantow M, Farkas SA, Malaisé M, Edtinger K, Shaotang Z, Koehl GE, Dahlke MH, Schlitt HJ, Geissler EK, Kroemer A. Double-deficiency for RORγt and T-bet drives Th2-mediated allograft rejection in mice. J Immunol 2013 191:4440-4446.
Safinia N., J. Leech, M. Hernandez-Fuentes, R. Lechler, G. Lombardi. Promoting transplantation tolerance: adoptive regulatory T cell therapy. Clin Exp Immunol. 2013 172: 158-168.
Scheffold A. How can the latest technologies advance cell therapy manufacturing?
Curr Opin Organ Transplant. 2013 Aug;18(4):408-15. Doi: 10.1097/MOT.0000000000000133
Scottà C., M. Esposito, H. Fazekasova, G. Fanelli, C.E. Francis, N. Ali, F. Xiao, M. Peakman, B. Afzali, P. Sagoo, R.I. Lechler*, G. Lombardi*. Differential effects of rapamycin and retinoic acid on expansion, stability and suppressive qualities of human CD4+CD25+FOXP3+ Treg subpopulations. Heamatologica. 2013 98:1291-1299.
Streitz M, Miloud T, Kapinsky M, Reed MR, Magari R, Geissler EK, Hutchinson JA, Vogt K, Schlickeiser S, Kverneland AH, Meisel C, Volk HD, Sawitzki B.Standardization of whole blood immune phenotype monitoring for clinical trials: panels and methods from the ONE study. Transplant Res. 2013 2(1):17.
Tang Q, Bluestone JA. Regulatory T-cell therapy in transplantation: moving to the clinic. Cold Spring Harb Perspect Med. 2013 Nov 1;3(11)
Volk HD, Sawitzki B, Reinke P. Molecular analysis of renal allograft biopsies--more than a nice toy for researchers? Am J Transplant. 2013 Mar;13(3):539-40. doi: 10.1111/ajt.12081.
Viklicky O1, Krystufkova E, Brabcova I, Sekerkova A, Wohlfahrt P, Hribova P, Wohlfahrtova M, Sawitzki B, Slatinska J, Striz I, Volk HD, Reinke P. B-cell-related biomarkers of tolerance are up-regulated in rejection-free kidney transplant recipients. Transplantation. 2013 Jan 15;95(1):148-54. doi: 10.1097/TP.0b013e3182789a24.
2012
Broichhausen C, Riquelme P, Geissler EK, Hutchinson JA.
Regulatory macrophages as therapeutic targets and therapeutic agents in solid organ transplantation. Curr Opin Organ Transplant. 2012 17(4):332-42.
Canavan J.B., B. Afzali, C. Scotta, H. Fazekasova, F.C. Edozi, T.T MacDonald, M.P. Hernadez-Fuentes, G. Lombardi, G. Lord. A rapid diagnostic test for human regulatory T cell function to enable regulatory T cell therapy. Blood. 2012 119(8):e57-66.
Eggenhofer E. and Hoo M. J Mesenchymal stem cell-educated Macrophages Transplant Res. 2012, 1:12
Geissler E. K. The ONE Study compares cell therapy products in organ transplantation: introduction to a review series on suppressive monocyte-derived cells Transplant Res. 2012, 1:11
Haynes L.D., Jankowska-Gan E., Sheka A., Keller M., Hernandez-Fuentes M., Lechler R.I., Seyferyt-Margolis V., Turka L.A., Newell K.A., Burlingham, W.J. Donor-Specific Indirect Pathway Analysis Reveals a B cell-Independent Signature which Reflects Outcomes in Kidney Transplant Recipients. Am J Transplant. 2012 Mar;12(3):640-8.
Jankowska-Gan E., Sheka A., Sollinger H.W., Pirsch J.D., Hofmann M.R., Haynes L.D., Armbrust M.J., Mezrich J.D., Burlingham W.J. Pre-transplant immune regulation predicts allograft outcome: bidirectional regulation correlates with excellent renal transplant function in living-related donor-recipient pairs. Transplantation 2012 Feb 15;93(3):283-90.
Issa F, Wood KJ. Translating tolerogenic therapies to the clinic - where do we stand? Front Immunol. 2012;3:254. Epub 2012 Aug 20.
Moreau A, Varey E, Bouchet-Delbos L, Cuturi M C Cell therapy using tolerogenic dendritic cells in transplantation Transplant Res. 2012, 1:13
Moreau A, Varey E, Bériou G, Hill M, Bouchet-Delbos L, Segovia M, Cuturi MC.
Tolerogenic dendritic cells and negative vaccination in transplantation: from rodents to clinical trials. Front Immunol. 2012 9;3:218
Olson BM, Jankowska-Gan E, Becker JT, Vignali DA, Burlingham WJ, McNeel DG. Human Prostate Tumor Antigen-Specific CD8+ Regulatory T Cells Are Inhibited by CTLA-4 or IL-35 Blockade. J Immunol. 2012 189:5590-601.
Riquelme P, Geissler E K, Hutchinson J A Alternative approaches to myeloid suppressor cell therapy in transplantation: comparing regulatory macrophages to tolerogenic DCs and MDSCs Transplant Res. 2012, 1:17
Schliesser U, Streitz M, Sawitzki B. Tregs: application for solid-organ transplantation. Curr Opin Organ Transplant. 2012 Feb;17(1):34-41.
Schoenbrunn A, Kohler S, Frentsch S, Keye J, Dooms H, Moewes B, Dong J, Loddenkemper C, Sieper J, Wu P, Romagnani C, Matzmohr N, Thiel A. A converse 4-1BB and CD40L expression pattern delineates activated Treg and Tcon enabling direct isolation of alloantigen-reactive natural Foxp3+ Treg J Immunol. 2012, 189:5985-5994.
Wood KJ, Bushell A, Hester J. Regulatory immune cells in transplantation. Nat Rev Immunol. 2012 May 25;12(6):417-30.
2011
Afzali B, P. J. Mitchell, C. Scottà, J. Canavan, F.C. Edozie, H. Fraser, S. John, L. Barber, M. Hernandez-Fuentes, R. I. Lechler*, and G. Lombardi* Relative resistance of human CD4+ memory T cells to suppression by CD4+CD25+ regulatory T cells. Am J Transpl. 2011. 11: 1734-1742.
Hutchinson JA, Riquelme P, Sawitzki B, Tomiuk S, Miqueu P, Zuhayra M, Oberg HH, Pascher A, Lützen U, Janssen U, Broichhausen C, Renders L, Thaiss F, Scheuermann E, Henze E, Volk HD, Chatenoud L, Lechler RI, Wood KJ, Kabelitz D, Schlitt HJ, Geissler EK, Fändrich F. Cutting Edge: Immunological consequences and trafficking of human regulatory macrophages administered to renal transplant recipients. J Immunol. 2011, 187(5):2072-8. Epub 2011 Jul 29.McMurchy AN, Bushell A, Levings MK, Wood KJ. Moving to tolerance: clinical application of T regulatory cells. Semin Immunol. 2011, 23(4):304-13. Epub 2011 May 28.
Wege, A., W. Ernst, J. Eckl, B. Frankenberger, A. Vollmann-Zwerenz, D.N. Männel, O. Ortmann, A. Kroemer*, G. Brockhoff. Humanized tumor mice - A new model to study and manipulate the immune response in advanced cancer therapy. Int J Cancer 2011, 129(9):2194-2206.
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